| Over the years, the global pharmaceutical company | | | | screening by Shi Yong Huo cell basis as a drug |
| focused on research and development on the faster | | | | discovery to address this issue, therefore, the |
| and better develop the technology for clinical drugs | | | | technology of another name is cell-based screening. |
| are very interested, and whom continue to work | | | | High-content drug screening defining characteristic is |
| very hard. Unfortunately, the discovery process was | | | | not observed drug candidates at a particular time |
| considered to be more reasonable and more | | | | instant on the subject of the role of a single target, |
| effective technology is not able to achieve its original | | | | but the use of a single instrument can work in a |
| vision. The fact is, despite the annual increase in the | | | | space and time, number of cells within the tracking |
| cost of drug development, but the real into the | | | | process. The process involves a protein changes, |
| market without a commensurate number of new | | | | such as G-protein coupled receptor (GPCR) signal |
| products known for. This makes a number of | | | | transduction, kinase-mediated signal transduction and |
| pharmaceutical companies have to rethink the way in | | | | ion channel signal transduction. For example, GPCR |
| what to filter the drug, while considering whether to | | | | activation can stimulate them into the cell. As |
| intervene directly to the development of the whole | | | | mentioned earlier, this process is visible, thus providing |
| process. | | | | a specific objective to measure the efficiency of |
| | | | | drug candidates. |
| High-throughput screening of Solomon | | | | |
| | | | | Cell electrolysis spectrum rivalry |
| Of the last century until the mid-90s, drug discovery | | | | |
| is mainly through high-throughput screening (HTS). Be | | | | Although the original high-content drug screening |
| regarded as the original high-throughput screening of | | | | identified by the fluorescence determination, but |
| various heavy pharmacological activity in vitro and in | | | | other technologies such as cell electrolysis measured |
| vivo experiments a reasonable substitute, which is | | | | spectrum appears, for some processes provided a |
| heavily dependent on liquid handling robotic devices, | | | | better choice. It does not require agents or other |
| automated test equipment and computer control to | | | | types of fluorescent markers for cell marking, and |
| study the new interest in biological target molecules | | | | can also detect a number of cell signaling channel. At |
| and between interaction. And high-throughput | | | | present, this technology is relatively low flux limit for |
| screening is used to match the so-called combinatorial | | | | the occasion, but the equipment manufacturers to |
| chemistry. | | | | increase throughput. In fact, the high content drug |
| | | | | screening can also be used for nucleic acid-based |
| Combinatorial chemistry method excludes the study | | | | processes, such as DNA transcription or RNA |
| of individual compounds, the traditional method, and | | | | interference, as well as cellular processes such as |
| replaced the same time of hundreds or even | | | | apoptosis, DNA damage repair or cell division occurs. |
| thousands of laboratory synthesized and stored in a | | | | Obviously, these cases require different method. For |
| large database of compounds. Combinatorial | | | | example, can be assayed for DNA degradation |
| chemistry methods De Chu Xian, gave birth to the | | | | assessment of apoptosis. |
| birth of new companies, these companies only | | | | |
| mission is to create a combinatorial library, and then | | | | High content drug screening is facing a major |
| transferred to the Drug Discovery, Gong interest of | | | | problem, because it is a kind of image-based |
| these companies target Jinxingshaixuan. Therefore, if | | | | technology, it can produce large amounts of data. For |
| there is no high-throughput screening technology, can | | | | example, a single experiment can generate up to |
| not be such a huge number of compounds screened, | | | | 10TB [trillion bytes; 1TB = 1000GB] data. Currently, |
| the application of high throughput screening process | | | | vendors are developing to deal with PB level (1PB = |
| to further promote the development of other | | | | 1000TB) quantitative data equipment, all these data |
| aspects of progress. | | | | must be processed and stored, Xianran this Yaoqiu |
| | | | | high content of drug screening have vast computing |
| It is worth mentioning that the completion of human | | | | power. Of course there are many other challenges, |
| genome projects, technological advances, such as | | | | such as the need to develop new methods to |
| more compact (from the original 96-well plates, | | | | measure a particular cell type of interest (such as |
| grown to 384,1536 and even 456 well plates), but | | | | immune system cells) in the process. |
| also help maintain the HTS technology as the main | | | | |
| driving force for drug discovery . Other technological | | | | Nevertheless, the advantages of high content drug |
| advances (including the new sense of technology) | | | | screening seems better than its shortcomings. The |
| such as quantitative protein drug candidates, when | | | | most significant advantage is that, compared with the |
| combined with changes in the differential scanning | | | | traditional determination, it was more useful |
| calorimetry or light scattering measurements. | | | | information. High content screening data, the |
| | | | | composition of space and time means that it studies |
| However, looking for new active molecules, the | | | | than the traditional one-dimensional HTS data more |
| production of high-throughput screening is difficult to | | | | biological significance, and can draw from the |
| cope with the large number of additional data, it is | | | | relationship between morphological changes. High |
| not more than the needle in a haystack. This allows | | | | content drug screening Another important attraction |
| pharmaceutical companies to squeeze them in many | | | | is its economy, especially in the current global financial |
| high-throughput screening program. For the | | | | crisis, this is an important consideration. It can lower |
| pharmaceutical industry, possible alternative strategy | | | | the cost of traditional methods to obtain large |
| is to acquire permits and development of similar | | | | amounts of information. In addition, the high content |
| drugs, but the technical terms are likely to turn high | | | | drug screening is also used in the discovery process |
| content screening (HCS). | | | | toxicological data early is crucial to help the |
| | | | | development of the drug candidates have the |
| High-content drug screening is essentially a simplified | | | | problem sorted and toxicity of compounds removed |
| method for drug discovery, but it is about a specific | | | | as soon as possible to reduce the subsequent |
| molecule with a specific receptor or enzyme | | | | demand for animal testing, due to high content drug |
| interactions can be very effective there. For | | | | screening is based on whole-cell research, so it can |
| example, receptors and enzymes can not exist in | | | | generate all animal experiments can only be provided |
| isolation, but they are complex cellular responses and | | | | by the data. |
| interaction part of the environment, high-content drug | | | | |